منابع مشابه
critical period effects in foreign language learning:the influence of maturational state on the acquisition of reading,writing, and grammar in english as a foreign language
since the 1960s the age effects on learning both first and second language have been explored by many linguists and applied linguists (e.g lennerberg, 1967; schachter, 1996; long, 1990) and the existence of critical period for language acquisition was found to be a common ground of all these studies. in spite of some common findings, some issues about the impacts of age on acquiring a second or...
15 صفحه اولBiochemistry a - Factor - directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae ( epidermal growth factor / protein fusion / peptide processing / yeast mating type / in vitro mutagenesis )
Saccharomyces cerevisiae cells were transformed with plasmids containing hybrid genes in which the sequence encoding mature human epidermal growth factor was joined to sequences encoding the leader region (preprosegment) of the precursor of the yeast mating pheromone afactor. These cells accurately process the hybrid protein and efficiently secrete authentic biologically active human epidermal ...
متن کاملAn Efficient Genome-Wide Fusion Partner Screening System for Secretion of Recombinant Proteins in Yeast
To produce rarely secreted recombinant proteins in the yeast Saccharomyces cerevisiae, we developed a novel genome-wide optimal translational fusion partner (TFP) screening system that involves recruitment of an optimal secretion signal and fusion partner. A TFP library was constructed from a genomic and truncated cDNA library by using the invertase-based signal sequence trap technique. The eff...
متن کاملNetworking proteins in yeast.
T advent of genome sequencing projects—culminating in the recent reports of the human sequence (1, 2)—has resulted in both the identification of novel genes and proteins as well as the proliferation of the ‘‘omes’’ that come from their analyses: the proteome (the complement of proteins), transcriptome (the complement of mRNA transcripts), metabolome (the complement of metabolites), and so on. T...
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ژورنال
عنوان ژورنال: Kagaku To Seibutsu
سال: 1988
ISSN: 0453-073X,1883-6852
DOI: 10.1271/kagakutoseibutsu1962.26.568